25-OH Vitamin D ELISA Kit

Produktnummer ABIN7831163

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human 25-OH Vitamin D.

Kurzübersicht für 25-OH Vitamin D ELISA Kit (ABIN7831163)

Target: 25-OH Vitamin D (25-OH-D)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Proben: Plasma, Serum

Güteklasse: QuickTest

Produktdetails 25-OH Vitamin D ELISA Kit

Verwendungszweck: FastELISA 25-OH-D (Human) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of Human 25-OH-D (25-Hydroxyvitamin D) in Serum, plasma.

Analytische Methode: Quantitative

Spezifität: This assay is highly specific for detection of Human 25-OH-D. No significant cross-reactivity or interference between Human 25-OH-D and analogues was observed.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Haltbarkeit: 6 months

Details zu 25-OH Vitamin D

Target: 25-OH Vitamin D (25-OH-D)

Andere Bezeichnung: 25-OH-D

Substanzklasse: Chemical