Cortisol ELISA Kit
Kurzübersicht für Cortisol ELISA Kit (ABIN7831122)
Target
Alle Cortisol ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
Güteklasse
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Verwendungszweck
- FastELISA Cortisol (Canine) ELISA Kit is a competitive enzyme immunoassay for the quantitative measurement of Canine Cortisol in serum, plasma.
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Analytische Methode
- Quantitative
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Cortisol ELISA Kit
Reaktivität: Diverse Spezies Colorimetric Sandwich ELISA Fecal, Hair, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReaktivität: Diverse Spezies Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Maus Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Diverse Spezies Colorimetric Sandwich ELISA 100 pg/mL - 3200 pg/mL Fecal, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Culture Medium, Urine
Cortisol ELISA KitReaktivität: Diverse Spezies Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum, Urine
Cortisol ELISA KitReaktivität: Human Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Rind (Kuh) Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Affe Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Pferd Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
Cortisol ELISA KitReaktivität: Schwein Colorimetric Competition ELISA 0.391 ng/mL - 25 ng/mL Plasma, Serum, Tissue Homogenate
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Pre-coated
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Protokoll
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Handhabung
- Avoid repeated freezing and thawing
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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Haltbarkeit
- 6 months
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- Cortisol
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Substanzklasse
- Hormone
Target Alle Cortisol ELISA Kits anzeigen
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