TACC1 ELISA Kit

Produktnummer ABIN7831054

Human TACC1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human TACC1.

Kurzübersicht für TACC1 ELISA Kit (ABIN7831054)

Target: TACC1 (Transforming, Acidic Coiled-Coil Containing Protein 1 (TACC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails TACC1 ELISA Kit

Verwendungszweck: TACC1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TACC1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu TACC1

Target: TACC1 (Transforming, Acidic Coiled-Coil Containing Protein 1 (TACC1))

Andere Bezeichnung: TACC1

Hintergrund:

Synonyms: DKFZp686K18126|Ga55|KIAA1103

Gene Description: transforming, acidic coiled-coil containing protein 1

Gen-ID: 6867

UniProt: O75410