TRIM3 ELISA Kit

Produktnummer ABIN7831052

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human TRIM3.

Kurzübersicht für TRIM3 ELISA Kit (ABIN7831052)

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails TRIM3 ELISA Kit

Verwendungszweck: TRIM3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TRIM3 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu TRIM3

Target: TRIM3 (Tripartite Motif Containing 3 (TRIM3))

Andere Bezeichnung: TRIM3

Hintergrund:

Synonyms: BERP|FLJ16135|HAC1|RNF22|RNF97

Gene Description: tripartite motif-containing 3

Gen-ID: 10612

UniProt: O75382