DKC1 ELISA Kit

Produktnummer ABIN7831049

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human DKC1.

Kurzübersicht für DKC1 ELISA Kit (ABIN7831049)

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails DKC1 ELISA Kit

Verwendungszweck: DKC1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DKC1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu DKC1

Target: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Andere Bezeichnung: DKC1

Hintergrund:

Synonyms: CBF5|DKC|FLJ97620|NAP57|NOLA4|XAP101

Gene Description: dyskeratosis congenita 1, dyskerin

Gen-ID: 1736

UniProt: O60832

Pathways: Telomere Maintenance