LMX1B ELISA Kit

Produktnummer ABIN7831047

Das Human LMX1B ELISA Kit (ABIN7831047) ist ein Colorimetric ELISA Kit zur Detektion von Human LMX1B.

Kurzübersicht für LMX1B ELISA Kit (ABIN7831047)

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails LMX1B ELISA Kit

Verwendungszweck: LMX1B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LMX1B expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu LMX1B

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Andere Bezeichnung: LMX1B

Hintergrund:

Synonyms: LMX1.2|MGC138325|MGC142051|NPS1

Gene Description: LIM homeobox transcription factor 1, beta

Gen-ID: 4010

UniProt: O60663

Pathways: Dopaminergic Neurogenesis