AKAP10 ELISA Kit

Produktnummer ABIN7831039

Human AKAP10 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human AKAP10.

Kurzübersicht für AKAP10 ELISA Kit (ABIN7831039)

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails AKAP10 ELISA Kit

Verwendungszweck: AKAP10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKAP10 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu AKAP10

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Andere Bezeichnung: AKAP10

Hintergrund:

Synonyms: D-AKAP2|MGC9414|PRKA10

Gene Description: A kinase (PRKA) anchor protein 10

Gen-ID: 11216

UniProt: O43572

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling