MAP3K13 ELISA Kit

Produktnummer ABIN7831036

Human MAP3K13 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MAP3K13.

Kurzübersicht für MAP3K13 ELISA Kit (ABIN7831036)

Target: MAP3K13 (Mitogen-Activated Protein Kinase Kinase Kinase 13 (MAP3K13))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MAP3K13 ELISA Kit

Verwendungszweck: MAP3K13 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAP3K13 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu MAP3K13

Target: MAP3K13 (Mitogen-Activated Protein Kinase Kinase Kinase 13 (MAP3K13))

Andere Bezeichnung: MAP3K13

Hintergrund:

Synonyms: LZK|MGC133196

Gene Description: mitogen-activated protein kinase kinase kinase 13

Gen-ID: 9175

UniProt: O43283

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2