CLOCK ELISA Kit

Produktnummer ABIN7831035

Das Human CLOCK ELISA Kit (ABIN7831035) ist ein Colorimetric ELISA Kit zur Detektion von Human CLOCK.

Kurzübersicht für CLOCK ELISA Kit (ABIN7831035)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CLOCK ELISA Kit

Verwendungszweck: CLOCK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CLOCK expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Andere Bezeichnung: CLOCK

Hintergrund:

Synonyms: KAT13D|KIAA0334|bHLHe8

Gene Description: clock homolog (mouse)

Gen-ID: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism