NDC80 ELISA Kit

Produktnummer ABIN7831026

Das Human NDC80 ELISA Kit (ABIN7831026) ist ein Colorimetric ELISA Kit zur Detektion von Human NDC80.

Kurzübersicht für NDC80 ELISA Kit (ABIN7831026)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NDC80 ELISA Kit

Verwendungszweck: NDC80 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NDC80 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Andere Bezeichnung: NDC80

Hintergrund:

Synonyms: HEC|HEC1|KNTC2|TID3|hsNDC80

Gene Description: NDC80 homolog, kinetochore complex component (S. cerevisiae)

Gen-ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location