VAV3 ELISA Kit

Produktnummer ABIN7831011

Das Human VAV3 ELISA Kit (ABIN7831011) ist ein Colorimetric ELISA Kit zur Detektion von Human VAV3.

Kurzübersicht für VAV3 ELISA Kit (ABIN7831011)

Target: VAV3 (Vav 3 Oncogene (VAV3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails VAV3 ELISA Kit

Verwendungszweck: VAV3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VAV3 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Andere Bezeichnung: VAV3

Hintergrund:

Synonyms: FLJ40431

Gene Description: vav 3 guanine nucleotide exchange factor

Gen-ID: 10451

Pathways: Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung