RRN3 ELISA Kit

Produktnummer ABIN7831005

Das Human RRN3 ELISA Kit (ABIN7831005) ist ein Colorimetric ELISA Kit zur Detektion von Human RRN3.

Kurzübersicht für RRN3 ELISA Kit (ABIN7831005)

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails RRN3 ELISA Kit

Verwendungszweck: RRN3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TIF-IA expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu RRN3

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Andere Bezeichnung: RRN3

Hintergrund:

Synonyms: DKFZp566E104|MGC104238|TIFIA

Gene Description: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)

Gen-ID: 54700

UniProt: Q9NYV6

Pathways: Negative Regulation of intrinsic apoptotic Signaling