NME2 ELISA Kit

Produktnummer ABIN7830985

Human NME2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NME2.

Kurzübersicht für NME2 ELISA Kit (ABIN7830985)

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NME2 ELISA Kit

Verwendungszweck: NME2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NM23 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu NME2

Target: NME2 (Nucleoside diphosphate kinase B (NME2))

Andere Bezeichnung: NME2

Hintergrund:

Synonyms: MGC111212|NDPK-B|NDPKB|NM23-H2|NM23B|puf

Gene Description: non-metastatic cells 2, protein (NM23B) expressed in

Gen-ID: 4831

UniProt: P22392