NEK9 ELISA Kit

Produktnummer ABIN7830984

Das Human NEK9 ELISA Kit (ABIN7830984) ist ein Colorimetric ELISA Kit zur Detektion von Human NEK9.

Kurzübersicht für NEK9 ELISA Kit (ABIN7830984)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NEK9 ELISA Kit

Verwendungszweck: NEK9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NEK9 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Andere Bezeichnung: NEK9

Hintergrund:

Synonyms: DKFZp434D0935|MGC138306|MGC16714|NERCC|NERCC1|Nek8

Gene Description: NIMA (never in mitosis gene a)- related kinase 9

Gen-ID: 91754

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interaktom, Phosphorylierungen bei SARS-CoV-2 Infektion