STK3 ELISA Kit

Produktnummer ABIN7830983

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human STK3.

Kurzübersicht für STK3 ELISA Kit (ABIN7830983)

Target: STK3 (serine/threonine Kinase 3 (STK3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails STK3 ELISA Kit

Verwendungszweck: STK3/STK4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Mst1/2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu STK3

Target: STK3 (serine/threonine Kinase 3 (STK3))

Andere Bezeichnung: STK3

Hintergrund:

Synonyms: FLJ90748|KRS1|MST2

Gene Description: serine/threonine kinase 3 (STE20 homolog, yeast)

Gen-ID: 6788

UniProt: Q13188

Pathways: Tube Formation