RPH3A ELISA Kit

Produktnummer ABIN7830971

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human RPH3A.

Kurzübersicht für RPH3A ELISA Kit (ABIN7830971)

Target: RPH3A (Rabphilin 3A (RPH3A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails RPH3A ELISA Kit

Verwendungszweck: RPH3A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPH3A expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu RPH3A

Target: RPH3A (Rabphilin 3A (RPH3A))

Andere Bezeichnung: RPH3A

Hintergrund:

Synonyms: KIAA0985

Gene Description: rabphilin 3A homolog (mouse)

Gen-ID: 22895

Pathways: Synaptic Vesicle Exocytosis