PKD2 ELISA Kit

Produktnummer ABIN7830970

Human PKD2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PKD2.

Kurzübersicht für PKD2 ELISA Kit (ABIN7830970)

Target: PKD2 (Protein Kinase D2 (PKD2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails PKD2 ELISA Kit

Verwendungszweck: PRKD2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKD2 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PKD2

Target: PKD2 (Protein Kinase D2 (PKD2))

Andere Bezeichnung: PRKD2

Hintergrund:

Synonyms: HSPC187|PKD2

Gene Description: protein kinase D2

Gen-ID: 25865

Pathways: cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity