PKC mu ELISA Kit

Produktnummer ABIN7830969

Das Human PKC mu ELISA Kit (ABIN7830969) ist ein Colorimetric ELISA Kit zur Detektion von Human PKC mu.

Kurzübersicht für PKC mu ELISA Kit (ABIN7830969)

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails PKC mu ELISA Kit

Verwendungszweck: PRKD1/PRKD2/PRKD3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKD1/PRKD2/PRKD3 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PKC mu

Target: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Andere Bezeichnung: PRKD1

Hintergrund:

Synonyms: PKC-MU|PKCM|PKD|PRKCM

Gene Description: protein kinase D1

Gen-ID: 5587

Pathways: Myometrial Relaxation and Contraction