NCOR1 ELISA Kit

Produktnummer ABIN7830959

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human NCOR1.

Kurzübersicht für NCOR1 ELISA Kit (ABIN7830959)

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NCOR1 ELISA Kit

Verwendungszweck: NCOR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NCOR1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu NCOR1

Target: NCOR1 (Nuclear Receptor Co-Repressor 1 (NCOR1))

Andere Bezeichnung: NCOR1

Hintergrund:

Synonyms: KIAA1047|MGC104216|N-CoR|TRAC1|hCIT529I10|hN-CoR

Gene Description: nuclear receptor co-repressor 1

Gen-ID: 9611

Pathways: Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process