Splicing Factor 1 ELISA Kit

Produktnummer ABIN7830945

Das Human Splicing Factor 1 ELISA Kit (ABIN7830945) ist ein Colorimetric ELISA Kit zur Detektion von Human Splicing Factor 1.

Kurzübersicht für Splicing Factor 1 ELISA Kit (ABIN7830945)

Target: Splicing Factor 1 (SF1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Splicing Factor 1 ELISA Kit

Verwendungszweck: SF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SF1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Andere Bezeichnung: SF1

Hintergrund:

Synonyms: D11S636|ZFM1|ZNF162

Gene Description: splicing factor 1

Gen-ID: 7536

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location