Vitamin D Receptor ELISA Kit

Produktnummer ABIN7830937

Human Vitamin D Receptor ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Vitamin D Receptor.

Kurzübersicht für Vitamin D Receptor ELISA Kit (ABIN7830937)

Target: Vitamin D Receptor (VDR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Vitamin D Receptor ELISA Kit

Verwendungszweck: VDR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VDR expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Andere Bezeichnung: VDR

Substanzklasse: Chemical

Hintergrund:

Synonyms: NR1I1

Gene Description: vitamin D (1,25- dihydroxyvitamin D3) receptor

Gen-ID: 7421

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway