Tumor Protein p73 ELISA Kit

Produktnummer ABIN7830923

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Tumor Protein p73.

Kurzübersicht für Tumor Protein p73 ELISA Kit (ABIN7830923)

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Tumor Protein p73 ELISA Kit

Verwendungszweck: TP73 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TP73 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Tumor Protein p73

Target: Tumor Protein p73 (TP73) (Tumor Protein P73 (TP73))

Andere Bezeichnung: TP73

Hintergrund:

Synonyms: P73

Gene Description: tumor protein p73

Gen-ID: 7161

Pathways: Regulation of Cell Size, Positive Regulation of Response to DNA Damage Stimulus