TOP2B ELISA Kit

Produktnummer ABIN7830921

Das Human TOP2B ELISA Kit (ABIN7830921) ist ein Colorimetric ELISA Kit zur Detektion von Human TOP2B.

Kurzübersicht für TOP2B ELISA Kit (ABIN7830921)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails TOP2B ELISA Kit

Verwendungszweck: TOP2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TOP2B expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu TOP2B

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Andere Bezeichnung: TOP2B

Hintergrund:

Synonyms: TOPIIB|top2beta

Gene Description: topoisomerase (DNA) II beta 180 kDa

Gen-ID: 7155