PTH ELISA Kit

Produktnummer ABIN7830865

Human PTH ELISA Kit, Colorimetric Assay für die Quantifizierung von Human PTH.

Kurzübersicht für PTH ELISA Kit (ABIN7830865)

Target: PTH (Parathyroid Hormone (PTH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PTH ELISA Kit

Verwendungszweck: PTH (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTH expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PTH

Target: PTH (Parathyroid Hormone (PTH))

Andere Bezeichnung: PTH

Substanzklasse: Hormone

Hintergrund:

Synonyms: PTH1

Gene Description: parathyroid hormone

Gen-ID: 5741

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process