PKC theta ELISA Kit

Produktnummer ABIN7830858

Das Human PKC theta ELISA Kit (ABIN7830858) ist ein Colorimetric ELISA Kit zur Detektion von Human PKC theta.

Kurzübersicht für PKC theta ELISA Kit (ABIN7830858)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PKC theta ELISA Kit

Verwendungszweck: PRKCQ (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKCQ expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Andere Bezeichnung: PRKCQ

Hintergrund:

Synonyms: MGC126514|MGC141919|PRKCT|nPKC-theta

Gene Description: protein kinase C, theta

Gen-ID: 5588

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling