PML ELISA Kit

Produktnummer ABIN7830846

Das Human PML ELISA Kit (ABIN7830846) ist ein Colorimetric ELISA Kit zur Detektion von Human PML.

Kurzübersicht für PML ELISA Kit (ABIN7830846)

Target: PML (Promyelocytic Leukemia (PML))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PML ELISA Kit

Verwendungszweck: PML (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PML expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PML

Target: PML (Promyelocytic Leukemia (PML))

Andere Bezeichnung: PML

Hintergrund:

Synonyms: MYL|PP8675|RNF71|TRIM19

Gene Description: promyelocytic leukemia

Gen-ID: 5371

Pathways: p53 Signalweg, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus