Progesterone Receptor ELISA Kit

Produktnummer ABIN7830840

Das Human Progesterone Receptor ELISA Kit (ABIN7830840) ist ein Colorimetric ELISA Kit zur Detektion von Human Progesterone Receptor.

Kurzübersicht für Progesterone Receptor ELISA Kit (ABIN7830840)

Target: Progesterone Receptor (PGR)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Progesterone Receptor ELISA Kit

Verwendungszweck: PGR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PGR expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Progesterone Receptor

Target: Progesterone Receptor (PGR)

Andere Bezeichnung: PGR

Hintergrund:

Synonyms: NR3C3|PR

Gene Description: progesterone receptor

Gen-ID: 5241

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Smooth Muscle Cell Migration