NPM1 ELISA Kit

Produktnummer ABIN7830825

Das Human NPM1 ELISA Kit (ABIN7830825) ist ein Colorimetric ELISA Kit zur Detektion von Human NPM1.

Kurzübersicht für NPM1 ELISA Kit (ABIN7830825)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NPM1 ELISA Kit

Verwendungszweck: NPM1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NPM1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: NPM1

Hintergrund:

Synonyms: B23|MGC104254|NPM

Gene Description: nucleophosmin (nucleolar phosphoprotein B23, numatrin)

Gen-ID: 4869

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly