GRIN2B ELISA Kit

Produktnummer ABIN7830802

Human GRIN2B ELISA Kit, Colorimetric Assay für die Quantifizierung von Human GRIN2B.

Kurzübersicht für GRIN2B ELISA Kit (ABIN7830802)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails GRIN2B ELISA Kit

Verwendungszweck: GRIN2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRIN2B expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Andere Bezeichnung: GRIN2B

Hintergrund:

Synonyms: MGC142178|MGC142180|NMDAR2B|NR2B|hNR3

Gene Description: glutamate receptor, ionotropic, N-methyl D-aspartate 2B

Gen-ID: 2904

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity