p300 ELISA Kit

Produktnummer ABIN7830797

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human p300.

Kurzübersicht für p300 ELISA Kit (ABIN7830797)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails p300 ELISA Kit

Verwendungszweck: EP300 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EP300 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Andere Bezeichnung: EP300

Hintergrund:

Synonyms: KAT3B|p300

Gene Description: E1A binding protein p300

Gen-ID: 2033

Pathways: p53 Signalweg, Notch Signalweg, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size