MMP1 ELISA Kit

Produktnummer ABIN7830769

Human MMP1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MMP1.

Kurzübersicht für MMP1 ELISA Kit (ABIN7830769)

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MMP1 ELISA Kit

Verwendungszweck: MMP1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MMP1 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu MMP1

Target: MMP1 (Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))

Andere Bezeichnung: MMP1

Hintergrund:

Synonyms: CLG|CLGN

Gene Description: matrix metallopeptidase 1 (interstitial collagenase)

Gen-ID: 4312

UniProt: P03956