LAT ELISA Kit

Produktnummer ABIN7830734

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human LAT.

Kurzübersicht für LAT ELISA Kit (ABIN7830734)

Target: LAT (Linker For Activation of T Cells (LAT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails LAT ELISA Kit

Verwendungszweck: LAT (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LAT expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu LAT

Target: LAT (Linker For Activation of T Cells (LAT))

Andere Bezeichnung: LAT

Hintergrund:

Synonyms: LAT1|pp36

Gene Description: linker for activation of T cells

Gen-ID: 27040

UniProt: O43561

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung