KCNJ11 ELISA Kit

Produktnummer ABIN7830728

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human KCNJ11.

Kurzübersicht für KCNJ11 ELISA Kit (ABIN7830728)

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails KCNJ11 ELISA Kit

Verwendungszweck: KCNJ11 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KCNJ11 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Andere Bezeichnung: KCNJ11

Hintergrund:

Synonyms: BIR|HHF2|IKATP|KIR6.2|MGC133230|PHHI|TNDM3

Gene Description: potassium inwardly-rectifying channel, subfamily J, member 11

Gen-ID: 3767

UniProt: Q14654

Pathways: Negative Regulation of Hormone Secretion