Integrin beta 3 ELISA Kit

Produktnummer ABIN7830702

Das Human Integrin beta 3 ELISA Kit (ABIN7830702) ist ein Colorimetric ELISA Kit zur Detektion von Human Integrin beta 3.

Kurzübersicht für Integrin beta 3 ELISA Kit (ABIN7830702)

Target: Integrin beta 3 (ITGB3)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Integrin beta 3 ELISA Kit

Verwendungszweck: ITGB3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ITGB3 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Andere Bezeichnung: ITGB3

Hintergrund:

Synonyms: CD61|GP3A|GPIIIa

Gene Description: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

Gen-ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex