ILK ELISA Kit

Produktnummer ABIN7830699

Das Human ILK ELISA Kit (ABIN7830699) ist ein Colorimetric ELISA Kit zur Detektion von Human ILK.

Kurzübersicht für ILK ELISA Kit (ABIN7830699)

Target: ILK (Integrin-Linked Kinase (ILK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ILK ELISA Kit

Verwendungszweck: ILK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ILK expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ILK

Target: ILK (Integrin-Linked Kinase (ILK))

Andere Bezeichnung: ILK

Hintergrund:

Synonyms: DKFZp686F1765|P59

Gene Description: integrin-linked kinase

Gen-ID: 3611

UniProt: Q13418

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Smooth Muscle Cell Migration