GRK2 ELISA Kit

Produktnummer ABIN7830688

Human GRK2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human GRK2.

Kurzübersicht für GRK2 ELISA Kit (ABIN7830688)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails GRK2 ELISA Kit

Verwendungszweck: ADRBK1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ADRBK1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Andere Bezeichnung: ADRBK1

Hintergrund:

Synonyms: BARK1|BETA-ARK1|FLJ16718|GRK2

Gene Description: adrenergic, beta, receptor kinase 1

Gen-ID: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling