GRB10 ELISA Kit

Produktnummer ABIN7830686

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human GRB10.

Kurzübersicht für GRB10 ELISA Kit (ABIN7830686)

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails GRB10 ELISA Kit

Verwendungszweck: GRB10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRB10 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu GRB10

Target: GRB10 (Growth Factor Receptor-Bound Protein 10 (GRB10))

Andere Bezeichnung: GRB10

Hintergrund:

Synonyms: GRB-IR|Grb-10|IRBP|KIAA0207|MEG1|RSS

Gene Description: growth factor receptor-bound protein 10

Gen-ID: 2887

UniProt: Q13322

Pathways: Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2