Filamin A ELISA Kit

Produktnummer ABIN7830652

Human Filamin A ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Filamin A.

Kurzübersicht für Filamin A ELISA Kit (ABIN7830652)

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Filamin A ELISA Kit

Verwendungszweck: FLNA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FLNA expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Filamin A

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Andere Bezeichnung: FLNA

Hintergrund:

Synonyms: ABP-280|ABPX|DKFZp434P031|FLN|FLN1|FMD|MNS|NHBP|OPD|OPD1|OPD2

Gene Description: filamin A, alpha (actin binding protein 280)

Gen-ID: 2316

UniProt: P21333

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location