ESR2 ELISA Kit

Produktnummer ABIN7830637

Human ESR2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ESR2.

Kurzübersicht für ESR2 ELISA Kit (ABIN7830637)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ESR2 ELISA Kit

Verwendungszweck: ESR2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ESR2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: ESR2

Hintergrund:

Synonyms: ER-BETA|ESR-BETA|ESRB|ESTRB|Erb|NR3A2

Gene Description: estrogen receptor 2 (ER beta)

Gen-ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling