EIF2S1 ELISA Kit

Produktnummer ABIN7830619

Human EIF2S1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human EIF2S1.

Kurzübersicht für EIF2S1 ELISA Kit (ABIN7830619)

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails EIF2S1 ELISA Kit

Verwendungszweck: EIF2S1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EIF2S1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Andere Bezeichnung: EIF2S1

Hintergrund:

Synonyms: EIF-2|EIF-2A|EIF-2alpha|EIF2|EIF2A

Gene Description: eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa

Gen-ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C