UBR5 ELISA Kit

Produktnummer ABIN7830612

Das Human UBR5 ELISA Kit (ABIN7830612) ist ein Colorimetric ELISA Kit zur Detektion von Human UBR5.

Kurzübersicht für UBR5 ELISA Kit (ABIN7830612)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails UBR5 ELISA Kit

Verwendungszweck: UBR5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of UBR5 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Andere Bezeichnung: UBR5

Hintergrund:

Synonyms: DD5|EDD|EDD1|FLJ11310|HYD|KIAA0896|MGC57263

Gene Description: ubiquitin protein ligase E3 component n-recognin 5

Gen-ID: 51366

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway