IL-10RA ELISA Kit

Produktnummer ABIN7830601

Human IL-10RA ELISA Kit, Colorimetric Assay für die Quantifizierung von Human IL-10RA.

Kurzübersicht für IL-10RA ELISA Kit (ABIN7830601)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails IL-10RA ELISA Kit

Verwendungszweck: IL10RA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL10RA expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Andere Bezeichnung: IL10RA

Hintergrund:

Synonyms: CDW210A|HIL-10R|IL-10R1|IL10R

Gene Description: interleukin 10 receptor, alpha

Gen-ID: 3587

UniProt: Q13651

Pathways: Growth Factor Binding