HDAC7 ELISA Kit

Produktnummer ABIN7830570

Das Human HDAC7 ELISA Kit (ABIN7830570) ist ein Colorimetric ELISA Kit zur Detektion von Human HDAC7.

Kurzübersicht für HDAC7 ELISA Kit (ABIN7830570)

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails HDAC7 ELISA Kit

Verwendungszweck: HDAC7 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of HDAC7 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu HDAC7

Target: HDAC7 (Histone Deacetylase 7 (HDAC7))

Andere Bezeichnung: HDAC7

Hintergrund:

Synonyms: DKFZp586J0917|FLJ99588|HD7A|HDAC7A

Gene Description: histone deacetylase 7

Gen-ID: 51564

UniProt: Q8WUI4

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development