PARK7/DJ1 ELISA Kit

Produktnummer ABIN7830557

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PARK7/DJ1.

Kurzübersicht für PARK7/DJ1 ELISA Kit (ABIN7830557)

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PARK7/DJ1 ELISA Kit

Verwendungszweck: PARK7 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PARK7 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PARK7/DJ1

Target: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Andere Bezeichnung: PARK7

Hintergrund:

Synonyms: DJ-1|DJ1|FLJ27376|FLJ34360|FLJ92274

Gene Description: Parkinson disease (autosomal recessive, early onset) 7

Gen-ID: 11315

UniProt: Q99497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport