CSK ELISA Kit

Produktnummer ABIN7830532

Human CSK ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CSK.

Kurzübersicht für CSK ELISA Kit (ABIN7830532)

Target: CSK (C-Src tyrosine Kinase (CSK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CSK ELISA Kit

Verwendungszweck: CSK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CSK expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Andere Bezeichnung: CSK

Hintergrund:

Synonyms: MGC117393

Gene Description: c-src tyrosine kinase

Gen-ID: 1445

UniProt: P41240

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events