ICAM-3/CD50 ELISA Kit

Produktnummer ABIN7830487

Human ICAM-3/CD50 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human ICAM-3/CD50.

Kurzübersicht für ICAM-3/CD50 ELISA Kit (ABIN7830487)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ICAM-3/CD50 ELISA Kit

Verwendungszweck: ICAM3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ICAM3 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: ICAM3

Hintergrund:

Synonyms: CD50|CDW50|ICAM-R

Gene Description: intercellular adhesion molecule 3

Gen-ID: 3385

UniProt: P32942