CD19 ELISA Kit

Produktnummer ABIN7830481

Human CD19 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CD19.

Kurzübersicht für CD19 ELISA Kit (ABIN7830481)

Target: CD19 (CD19 Molecule (CD19))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CD19 ELISA Kit

Verwendungszweck: CD19 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD19 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CD19

Target: CD19 (CD19 Molecule (CD19))

Andere Bezeichnung: CD19

Hintergrund:

Synonyms: B4|MGC12802

Gene Description: CD19 Molecule

Gen-ID: 930

UniProt: P15391

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung