CCR5 ELISA Kit

Produktnummer ABIN7830480

Das Human CCR5 ELISA Kit (ABIN7830480) ist ein Colorimetric ELISA Kit zur Detektion von Human CCR5.

Kurzübersicht für CCR5 ELISA Kit (ABIN7830480)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CCR5 ELISA Kit

Verwendungszweck: CCR5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCR5 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CCR5

Hintergrund:

Synonyms: CC-CKR-5|CCCKR5|CD195|CKR-5|CKR5|CMKBR5|IDDM22

Gene Description: chemokine (C-C motif) receptor 5

Gen-ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size