JUP ELISA Kit

Produktnummer ABIN7830476

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human JUP.

Kurzübersicht für JUP ELISA Kit (ABIN7830476)

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails JUP ELISA Kit

Verwendungszweck: JUP (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JUP expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: JUP

Hintergrund:

Synonyms: ARVD12|CTNNG|DP3|DPIII|PDGB|PKGB

Gene Description: junction plakoglobin

Gen-ID: 3728

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location