CAMK4 ELISA Kit

Produktnummer ABIN7830464

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human CAMK4.

Kurzübersicht für CAMK4 ELISA Kit (ABIN7830464)

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CAMK4 ELISA Kit

Verwendungszweck: CAMK4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK4 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CAMK4

Target: CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))

Andere Bezeichnung: CAMK4

Hintergrund:

Synonyms: CaMK-GR|MGC36771

Gene Description: calcium/calmodulin-dependent protein kinase IV

Gen-ID: 814

UniProt: Q16566

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma